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Its applications vary from a nontoxic plasticizer to a preserv ative that prevents the clotting of stored blood First isolated from lemon juice by the Swedish chemist Carl Wilhelm Scheele in the late eighteenth century, pharmaceutical companies rst produced citric acid from lemons supplied by an Italian cartel For example, citric acid ex tracted from Italian citrus was P zer s most popular product for decades leading up to World War I However, the start of the war in 1914 inter rupted the importation of lemons by many of the citric acid producers in the United States and Europe By fortuitous accident, scientists at P zer who were working on the fermentation of cheese discovered, in 1917, that citric acid could be produced by fermentation Within a decade, the company was meeting all of its market demand for citric acid through fermentation-based production.

(~) 3

(8.4.44)

V(3) _

(8.4.45)

NOTE: You must run the unit test project without debugging (Debug | Start Without Debugging) in order to build the code coverage results

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where eiko ejk, and elk are unit vectors along the directions joining the center of particle k to the centers of particles i, j, and l, that form a triple-bond with particle k. A necessary condition is that the diameter of the inscribed circle within the triangle, whose vertices are the centers of particles i, j, and l, must be be less than the diameter of the test particle k, as shown in Fig.8.4.7c. The calculations of the total effective volumes ~~), a = 0,1,2,3 of the test particle k do not depend on its current binding state. In the trial move of sticky particles, the final move consists of changing the binding state. The binding state may change from its present ,a-bond configuration to a new ,a'-bond configuration. Thus, each move will involve a transition among zero bond, single bond, double bond, and triple bond states. We calculate ~~) and Ve~) by doing the following. We first make a list of all pairs of particles with which the test particle k can form a double-bond. This double-bond list is then used to compute the total effective volume ~~) according to (8.4.42). From the list of pairs we further select those triplets that can form a triple-bond with the test particle, and these possible triplets are used to calculate the total effective volume Ve~) using (8.4.45). In this algorithm, we let the test particle free to move over the entire volume, every particle, except itself, is possible to form a single-bond with the test particle. Thus the total effective volumes Ve~) and ~~) are constants during the whole simulation process. The new binding state (3' for the test particle is selected

P({3')

((3')

(8.4.46)

See the Additional Reading section for more information on all of the many features available when executing tests and reviewing results

Today, virtually all of the worlds production of citric acid on the or der of 400,000 metric tons is produced through fermentation of a fungus with food sources as varied as the syrup or waste from sugar cane, dates, sweet potatoes, and pineapples to bananas, potatoes, and wheat bran Al though the cost of food for the fermentation process is an important eco nomic factor, the cost of citric acid production is primarily determined by the ef ciency of the fungal metabolism Thus, the methodology used to se lect and maintain strains of fungi with highly ef cient metabolisms is a closely guarded secret.

the system prior to the particle k being moved The three-dimensional Monte Carlo simulation of adhesive particles begins with N unbounded spheres randomly or regularly placed inside a cubic box with no overlap In the first stage, the Monte Carlo steps are similar to the ones described in Section 831 for non-sticky particles The spheres are shuffled to create an initial realization In the second stage, however, instead of moving the test particle randomly within a small cube of side ~ to determine its trial location, the test adhesive sphere is displaced according to the breaking of its current binding state and the formation of new binding state with other particles Thus the second stage consists of transitions among binding states If the test particle is found to bind with four or more particles, it will not be moved.

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